Journal: Toxins

Article Title: Domperidone Inhibits Clostridium botulinum C2 Toxin and Bordetella pertussis Toxin.

doi: 10.3390/toxins15070412

Figure Lengend Snippet: Figure 5. DOM reduces the amount of ADP-ribosylated Gαi in cells without affecting PTS1 enzyme activity in vitro or binding of PT to cells. (a) CHO cells were pre-incubated with VER or DOM for 30 min at 37 ◦C. As the control, cells were left untreated (con). Then, cells were challenged with 10 ng/mL PT

Article Snippet: Samples were analyzed by SDS-PAGE and Western blotting, detecting PTS1 with a specific antibody (Santa Cruz, #sc-57639 (63.1G9)).

Techniques: Activity Assay, In Vitro, Binding Assay, Incubation, Control

Journal: Toxins

Article Title: Domperidone Inhibits Clostridium botulinum C2 Toxin and Bordetella pertussis Toxin.

doi: 10.3390/toxins15070412

Figure Lengend Snippet: Figure 6. In the presence of DOM, less free PTS1 is detected in cells. CHO cells were pre-incubated with the respective inhibitors for 30 min at 37 ◦C. As the control, cells were treated with DMSO, the solvent of inhibitors, or left untreated. PT (100 ng/mL) was added for 4 h. After washing, cells were fixed and permeabilized, and PTS1 was stained by incubation with a specific primary antibody followed by fluorescence-labeled secondary antibody. Nuclei were stained by Hoechst. Images were taken with Keyance fluorescence microscope. Green = PTS1; blue = nucleus. White squares indicate magnified area.

Article Snippet: Samples were analyzed by SDS-PAGE and Western blotting, detecting PTS1 with a specific antibody (Santa Cruz, #sc-57639 (63.1G9)).

Techniques: Incubation, Control, Solvent, Staining, Fluorescence, Labeling, Microscopy

Journal: Toxins

Article Title: Domperidone Inhibits Clostridium botulinum C2 Toxin and Bordetella pertussis Toxin.

doi: 10.3390/toxins15070412

Figure Lengend Snippet: Figure 7. Inhibitors reduce the chaperone-mediated increase in PTS1 enzyme activity. (a) Recombinant PTS1 (100 nM) was incubated at RT or 37 ◦C for 15 min. ATP (1 mM) was added for another 30 min

Article Snippet: Samples were analyzed by SDS-PAGE and Western blotting, detecting PTS1 with a specific antibody (Santa Cruz, #sc-57639 (63.1G9)).

Techniques: Activity Assay, Recombinant, Incubation